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Analogues d'ADN/ARN (pour les OSAs)
| DNA/RNA Analogs | Position | ||
| 5’ | 3’ | int | |
| 2’ OMe-A, 2’ OMe-C, 2’ OMe-G, 2’ OMe-U | X | ||
| 2’ MOE-A, 2’ MOE-5-MeC, 2’ MOE-G, 2’ MOE-T | X | ||
| 2’ F-dA, 2’ F-dC, 2’ F-dG, 2’ F-dU | X | ||
| LNA-A, LNA-C (5mC), LNA-G, LNA-T | X | ||
| S-cEt-A/C/G/T/U/5mC | X | ||
2’ OMe-A, 2’ OMe-C, 2’ OMe-G, 2’ OMe-U
The incorporation of 2'-O-Methyl RNA nucleotides induces a resistance to a wide variety of nucleases, in particular RNase. Furthermore, 2’-OMe oligonucleotides show slightly increased affinity towards their complementary mRNA target sequence, thereby forming more stable hybrid duplexes compared to their non-modified DNA or RNA counterparts. This enables the formation of more stable hybrids with complementary RNA strands than would be the case for non-modified DNA and RNA sequences.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | X |
X
|
X
|
X | X |
X
|
X |
2’ MOE-A, 2’ MOE-5-MeC, 2’ MOE-G, 2’ MOE-T
Oligonucleotides incorporating 2'-O-methoxyethyl (MOE)-modified nucleotides, can support most, if not all antisense mechanisms of action. Further key distinctive characteristics are nuclease resistance, lower toxicity, superior target binding specificity, as well as increased affinity towards complementary RNA. For more detailed information about 2'-MOE antisense oligonucleotides from Microsynth, please see the flyer under "Related Downloads".
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | X |
X
|
X
|
O | X |
X
|
X |
2’ F-dA, 2’ F-dC, 2’ F-dG, 2’ F-dU
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | X |
X
|
X
|
X | X |
X
|
X |
LNA-A, LNA-C (5mC), LNA-G, LNA-T
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int | X |
X
|
X
|
X |
X
|
X | |