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Custom DNA Oligonucleotides & PCR Primers

DNA Oligonucleotides
 
 
Reliable custom DNA oligonucleotides and PCR primers manufactured with advanced synthesis technology and stringent quality control — trusted by researchers and biotech companies.
 

Reliable DNA Oligo Synthesis for Research & Biotech

Microsynth provides custom DNA oligonucleotide synthesis tailored to the needs of academic research, biotechnology and diagnostic development.
Our highly automated and digitally integrated production ensures consistent quality, reproducibility and short lead times.

Whether you require standard desalted primers, HPLC-purified probes or complex dual-labeled oligos, we deliver reliable performance for sensitive downstream applications.

Applications include:

  • PCR and qPCR
  • Sanger sequencing primers
  • NGS library preparation
  • Genotyping and SNP analysis
  • Molecular diagnostics
  • Cloning and gene assembly

Why Choose Microsynth DNA Oligos?

Fast Turnaround Times
Optimized synthesis workflows and automated production enable short delivery times without compromising quality.

High Quality & Stringent QC
Each oligo undergoes rigorous quality control including:

  • Trityl monitoring during synthesis
  • MALDI-TOF or ESI mass spectrometry
  • Analytical HPLC (where applicable)

This ensures correct molecular weight, high purity and reliable experimental performance.

Broad Range of Modifications
We offer an extensive selection of 5’, 3’ and internal modifications, enabling advanced assay design and probe development.

Flexible Synthesis Scales
From small-scale research quantities to larger batch requirements — synthesis scales are adapted to your project needs.

Synthesis Scales

We offer five standard DNA oligo synthesis scales ranging from research quantities to high-yield production. Yield depends on sequence length and purification method (click on the blue beam for detailed tables).

Synthesis Scales
 
Desalted DNA Oligos
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics 13 - 60 2 10 1
0.04 µmol 13 - 80 3 15 1
0.2 µmol 6 - 150 10 50 1
1.0 µmol 6 - 80 50 250 1
15 µmol 13 -60 700 3'500 2
 
RP-HPLC Purified DNA Oligos 
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol 13 - 50 1 5 2
0.2 µmol 6 - 50 3 15 2
1.0 µmol 6 - 50 15 75 2
15 µmol 6 - 50 300 1'500 3
 
IEX-HPLC Purified DNA Oligos
Synthesis scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]5
Genomics not available
0.04 µmol 40 - 80 1 1.7 3-5
0.2 µmol 2 3.3 3-5
1.0 µmol 6 10 3-5
15 µmol not available
 
RP-HPLC Purified & Dialyzed DNA Oligos 
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol not available
0.2 µmol 8 - 50 3 15 3
1.0 µmol 8 - 50 15 75 3
15 µmol 8 - 50 200 1'000 4
 
PAGE Purified DNA Oligos
Synthesis Scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol 13 - 80 0.5 2.5 2
0.2 µmol

8 - 80

81 - 150

2

0.5

10

2.5

 2
1.0 µmol 8 - 80 7 35 2
15 µmol not available
 
 
1 The synthesis scale represents the initial amount of 3' bases (starting material).
2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20mer.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 DNA bases; it may vary for sequences with high GC contents >70% etc.
4 Yields indicated in nmol represent an example calculation for a 60 nt DNA Oligo. Formula: nmol= OD*100/length of the Oligo

Available DNA Modifications


Design highly customized oligos with a broad portfolio of 5’, 3’ and internal modifications.

To learn more about the key specifications of a modification, please look at Modifications.

If your desired modification is not listed please contact us for availability.

Quality Control & Manufacturing Standards

Our DNA oligos are produced using state-of-the-art synthesizers and controlled manufacturing processes.

Quality assurance includes:

  • Controlled synthesis chemistry
  • In-process monitoring
  • Final mass verification
  • Optional analytical documentation

This ensures batch-to-batch consistency and reliable experimental results.

How to Order

 
  • Enter our webshop
  • Click on DNA in the "DNA/RNA Synthesis" domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering )
  • Follow the further instructions

 

To obtain information about our prices, please login to our webshop and download the current price list or ask your sales manager for a specific quote (attractive discounts are available if you plan to order more regularly from Microsynth or if you have a volume order).