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Desalted

Purification
 
Oligonucleotides are assembled via solid phase synthesis. In that way, nucleotides are attached according to the sequence of choice leading to chain growth. It is generally accepted that chemical reactions never reach 100% conversion. In state-of-the art oligonucleotide synthesis coupling yields of up to 99.5% are achieved. With the unreacted 0.5% of the strands, chain assembly stops and truncated sequences form as side products.
Depending on the intended application, it is advantageous to remove these shorter sequences from the full-length product. Therefore, Microsynth offers various types of purification methods.


Overview

Desalted

All our oligos are at least desalted to largely remove residual low molecular by-products arising and accumulating from the frequent chemical reactions occurring during synthesis. Such purification is sufficient for oligonucleotides shorter than 30 nt and/or oligonucleotides that are used for non-critical applications, such as PCR, sequencing, probing, mobility shift or hybridization. However, desalted oligos are not recommended for use in molecular cloning projects.

Potential Applications:

  • PCR
  • DNA sequencing
  • Probing
  • Mobility shift or hybridization

DNA Yields

 
Desalted DNA Oligos
Synthesis scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics 13 - 60 2 10 1
0.04 µmol 13 - 80 3 15 1
0.2 µmol 6 - 1504 10 50 1
1.0 µmol 6 - 80 50 250 1
15 µmol 13 -60 700 3'500 2
 
 
 
1 The synthesis scale represents the initial amount of 3' bases (starting material).
2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20mer.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 DNA bases; it may vary for sequences with high GC contents >70% etc.
4 Oligos longer than 150 DNA bases on request (we would like to discuss the proposed experiment/application with you beforehand in order to guarantee the best possible outcome)
5 Yields indicated in nmol represent an example calculation for a 60 nt DNA Oligo. Formula: nmol= OD*100/length of the Oligo

RNA Yields


Desalted RNA Oligos 
Synthesis scale1 Length Restriction Guaranteed Yield2 Production Time [wd]
[OD260] [nmol]3
Genomics not available
0.04 µmol 10 - 30 4 21 2
0.2 µmol 10 - 50 8 35 2
1.0 µmol 10 - 50 18 80 2
15 µmol 10 - 40 400 1'800 3
 

 

1 The synthesis scale represents the initial amount of 3' bases (starting material).

2 Our guaranteed and average yields are measured in OD and are valid only for unmodified oligos >20 and <40 nucleotides.
3 Yields indicated in nmol represent an example calculation for a 20mer. For this calculation the following rule of thumb equation was applied: nmol of oligo = OD x 100/length of oligo. Please note that this calculation is based on sequences with virtually homogenous distribution of the 4 RNA bases.