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miCORE Resequencing

Whole Genome Sequencing
 
 
High-resolution mutation detection across entire genomes. miCORE resequencing provides accurate insights into SNPs, InDels, and structural variants with Illumina sequencing. Whether for CRISPR off-target validation, strain verification, or comparative genomics, our resequencing service delivers the data you need to study genetic variation at scale

What You Can Achieve

  • Detect inherited or acquired mutations, from SNPs to large structural variations
  • Compare genomes across strains, isolates, or individuals
  • Verify on-target edits and identify off-target mutations from CRISPR/Cas9 experiments
  • Confirm genetic modifications in breeding and cell line studies
  • Study genome-wide differences linked to disease or phenotype

Before You Start

To set up your resequencing project effectively, consider:

  • Scientific objective – mutation detection, genome comparison, CRISPR validation
  • Reference genome quality – completeness and annotation status
  • Sequencing depth – coverage needed for SNV and InDel calling
  • Read length – optimal for resolution of complex variants
  • Sample purity – avoid substantial DNA contamination

Our experts support you from experimental design to bioinformatics analysis.

Modular Workflow

Choose a full-service package or individual modules. A typical resequencing project includes:

Workflow Resequencing

Bioinformatics Analysis

Our resequencing analysis module provides a wide range of deliverables:

  • Data quality control
  • Alignment and genome coverage analysis
  • SNV and small InDel detection (VCF files)
  • Structural variation and copy number analysis
  • Annotation of variants with amino acid-level consequences (for annotated references)
  • Optional: background filtering, consensus sequence assembly, genomic epidemiology (prokaryotes)

Results are delivered in standard formats (.vcf, .bam, .bed, .html, .pdf) with summary reports for easy interpretation.

Example Results

All results are delivered in publication-ready figures and interactive formats, providing both high-level overviews and nucleotide-level detail.

Turnaround Time

  • Sequencing only: 20 working days
  • Data analysis (bioinformatics): +3 working days (small genomes) or +6 working days (large genomes)
  • Express service available upon request

Sample Requirements

  • DNA buffer: 10 mM Tris-HCl (pH 7.5–8.5)
  • Avoid: EDTA >1 mM
  • DNA quantification: Fluorometric methods (e.g., PicoGreen®, Qubit®)

Contact us for project-specific requirements regarding DNA amount and quality.