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NGS Adapters & Indexing Primers

DNA Oligos for Next Generation Sequencing Applications
 
 
The quality and purity of NGS adapters and indexing primers are critical for the success of every next generation sequencing project. Thanks to its vast experience in the area of oligonucleotide synthesis (which extends beyond 30 years) as well as next generation sequencing (for more than 10 years), Microsynth knows exactly what the key requirements are for NGS oligos. Therefore, by choosing Microsynth, you're guaranteed to be in good hands when requesting NGS oligos only, or equally when outsourcing your entire NGS project.

Features and Benefits

 
 
High Quality
  • Oligonucleotide manufacturing processes validated for NGS sequencing.
  • Stringent quality control and regular process validation
  • Microsynth has > 15 years of experience in NGS sequencing and -oligo manufacturing

 

Fast Production
  • 3 - 5 working days
 
Excellent Technical Support
  • Combined in-house expertise in oligo manufacturing and NGS sequencing.
User-friendly Online Ordering System
  • User-friendly online portal with a series of helpful tools (e.g. order tracking & history, convenient search and re-order option

 

 
 
Cost Effective
  • Very competitive pricing
  • High guaranteed yields

 

Convenient
  • Access to a wide range of current modifications (e.g., 5'-Phosphate, PTO, 5-Me-dC)
  • Customizable oligos tailored for NGS applications
  • Compatible with any sequencing platform or technology
  • Analytical HPLC provided (optional)
  • Certificate of Analysis provided (optional)
  • Flexible delivery formats (dried, liquid, tubes, 96-well plates, etc.)
  • Option to outsource the entire NGS project, from isolation to bioinformatics
 

Synthesis Scales

 

Synthesis Scale Purification Length Restriction Guaranteed Yield Production Time [wd]
0.2 umol  IEX-HPLC 40 – 80 2 OD 3 - 5
1.0 umol  IEX-HPLC 40 – 80 6 OD 3 - 5

Microsynth has established and validated a specific process for the preparation of NGS oligonucleotides, based on a rigorous HPLC purification procedure in which the elimination of cross-contamination is a top priority.

How to Order

 
 
Important: Please first contact us at Microsynth and request your offer (volume discounts are possible!)
 

Option 1: When ordering NGS primers, please proceed as follows:

  • Enter our webshop, click on DNA in the blue “DNA/RNA Synthesis” domain
  • Select either Normal Entry in order to type or copy/paste the desired sequence information etc. or alternatively select Upload Entry by using our convenient Excel Template (can be downloaded during ordering)
  • Follow the further instructions

 

Option 2: When ordering primers for Illumina amplicon sequencing, please proceed as follows:

  • Use the dedicated “Order Form_Illumina_AmpliconDeepSeq” (can be downloaded in the right column); in this context see also our application note “Amplicon Deep Sequencing”)
  • Specify now the locus-specific sequences for your first-step PCR primers and then select your desired indexed forward and reverse primers
  • Enter our webshop, click on DNA in the blue “DNA/RNA Synthesis” domain and then on Upload Entry
  • Follow the further instructions