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ONT Resequencing (Prokaryotic/Eukaryotic)

 
 
The ONT Resequencing service from Microsynth leverages the power of Oxford Nanopore’s long-read sequencing technology to deliver comprehensive insights into your samples. With capabilities to resolve complex regions and structural variations, this service is ideal for advanced genomic analysis in prokaryotes and eukaryotes.

 

Features and Benefits


Oxford Nanopore Technologies (ONT) offers unique advantages over traditional short-read sequencing methods such as Illumina. Key benefits include:

  • Ultra-Long Read Lengths: Reads up to 200 kb enable analysis of regions that are inaccessible with short-read sequencing.
  • Resolution of Structural Variations: Detect complex genomic rearrangements .
  • Resolution of Repetitive Regions: Detect regions with repetitive sequences.
  • Insertion Site or Knockout Verification
  • Versatility Across Genomes: Suitable for all organisms, including fungi, plants, and prokaryotes.

 

Workflow


Our ONT Resequencing workflow ensures high-quality results and includes the following steps:

DNA Isolation
For optimal results, high-molecular-weight (HMW) DNA is essential. Our team employs specialized protocols for challenging samples, such as those with cell walls (plants, fungi) or contaminants from secondary metabolites. We also support customer-provided isolation protocols to tailor the process to your needs.

DNA Library Preparation
Using the appropriate sequencing kit, we prepare libraries to maximize read lengths and sequencing efficiency.

Sequencing
With a recommended >30x read coverage, we ensure comprehensive genomic data acquisition. Custom sequencing depths can be discussed to balance data quality and cost-effectiveness.

Variant Calling Analysis
Our state-of-the-art bioinformatics pipelineidentifies:

  • Single nucleotide variants (SNVs) and small insertions/deletions (InDels)
  • Structural variations (SVs)
  • Annotated variants for downstream interpretation

Results


You will receive detailed results in various formats, including:

  • Comprehensive Tables and Graphs: similar to those provided in our BacterialSeq service, ensuring clarity and actionable insights.
  • Variant Analysis:
    • SNVs and small InDels
    • Structural variations (SVs)
    • Annotation of genomic variations

 

Turnaround Time

 
Results are delivered within 7 days (<30 Mb genome), 2 weeks (<750 Mb genome), or 3 weeks (<2 Gb genome) after receiving the samples.