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Sample Requirements
Please take some time to review our sample requirements. By following these guidelines you increase the quality of your results and you give us the possibility to help you further.
Concentration
| Template | Concentration |
| Plasmid | 40-100 ng/µl |
|
PCR (purified and unpurified products)*
PCR 1000 - 2000 bp
PCR 500 - 1000 bp
PCR 250 - 500 bp
PCR 100 - 250 bp
|
General Rule: 1.5 ng/µl per 100 bp
30 ng/µl
15 ng/µl
7.5 ng/µl
4 ng/µl
|
|
E.coli
|
50 µl LB Medium OR
visible colony on agar plate
|
| E.coli (Ecoli NightSeq®) |
add 1 colony (diameter 1 mm) OR
add 5 µl turbid liquid culture
|
| Plasmid (Full PlasmidSeq) |
20 ng/µl
|
| Genomic DNA (BacterialSeq) | 50 ng/µl |
*Upfront PCR purification is highly recommended but can easily be outsourced to Microsynth.
|
Primer
|
Concentration
|
| Premixed | 4 pmol/µl = 4 µM |
|
As separate tube (enclosed)
|
10 pmol/µl = 10 µM
|
Volumes
| Service | Volume | Additional Information |
|
Economy Run (Tube)
|
12 µl
|
Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
|
|
Economy Run (Plate)
|
10 µl/well in 96-well plate
|
Increase the volume by 5 µl /well for each additional reaction
|
|
Premium Run
|
At least 20 µl
|
Increase the volume by 5 µl for each additional reaction
|
|
Primer Walking Service
|
At least 20 µl
|
Single Stranded 0.5 µg of plasmid-DNA for every kb of your construct
Double stranded 1 µg of plasmid-DNA for every kb of your construct |
|
Full PlasmidSeq
|
At least 15 µl
|
Suitable plasmid sizes range from 2.5 kb to 25 kb. While plasmids ranging from 25 kB to 50 kb have not been tested yet, you may try them at your own risk. |
|
BacterialSeq
|
At least 20 µl
|
High Quality DNA is needed for this services. That means high-molecular weight DNA with no fragments <5 kb. |
| Primer | Volume |
| As separate tube (enclosed) | 20 µl + 3 µl per additional reaction |
Storage
|
Service
|
Storage at Microsynth |
|
Economy Run (Tube)
|
4 days
|
|
Economy Run (Plate)
|
1 month
|
|
Ecoli NightSeq® (Tube or Plate)
|
1 month
|
|
Premium Run
|
3 months
|
|
Primer Walking Service
|
3 months
|
|
Full PlasmidSeq (Tube)
|
4 days
|
|
Full PlasmidSeq (Plate)
|
1 month
|
|
BactSeq (Tube)
|
1 month
|
|
Primer (stored)
|
4 months
|
|
Primer (custom primer list)
|
10 months
|