Long PCRSeq

Use our drop box system, or send your samples by mail.

>15 µl at 20 ng/µl

We supply additional information that allow you to identify problematic regions and evaluate the overall sequencing quality:

For instance, in the *.uncertain.tsv file that opens with Excel, all residues of the contig are listed that show a rate >25% of mismatches, deletions and/or insertions. These values are based on all the reads that were used to create the contig. You may sort the list according to % mismatches, % deletions, or % insertions to identify the regions within your plasmid that might be problematic or ambiguous. The coverage, as well as base quality of the reads at the respective position are also indicated.
In the *.clean.reads.html file of the Sequencing subfolder of your package you can inspect the statistics of the reads that were used to assemble your sample. The statistics comprise number of reads, length of reads and quality score of reads.

If you are working on Windows you may want to switch on viewing of file name extensions (In File Explorer under View, in the Show/hide group, select the File name extensions check box). For instance tab separated value files (.tsv) may be opened in any text editor or by drag and drop in an open but empty Excel table. Fasta (.fasta) and genbank (.gbk) files may be opened in a text editor or by specialized software.

Depending on the sequencing chemistry used, the assembler may face challenges reconstructing the terminal ends of linear DNA, potentially leading to the absence of up to ~25 nucleotides at the 3’ and/or 5’ ends of your insert.