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Quenchers (for RNA)
|
Type of Modification
|
Position
|
Quenching Range
|
||
|
5’
|
3’
|
Int.
|
(nm)
|
|
|
X
|
480-580 / 560-670 | |||
Black Hole Quencher-1
BHQ-1 is best suited for fluorophores that emit in the wavelength range from FAM to TAMRA.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
|
5’
|
O | O |
O
|
|
|||
|
3’
|
|
X
|
X
|
O
|
|
X
|
X
|
X = available/on stock
O = on request
Black Hole Quencher-2
BHQ-2 is best suited for fluorophores in the Cy3 to Cy5 wavelength range.
|
Position
|
Synthesis Scale
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
|
5’
|
O | O |
O
|
|
|||
|
3’
|
|
X
|
X
|
O
|
|
X
|
X
|
X = available/on stock
O = on request