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Exigences par rapport aux échantillons
Veuillez prendre un peu de temps pour examiner nos exigences en matière d'échantillons. En suivant ces recommandations, vous améliorez la qualité de vos résultats et vous nous donnez la possibilité de vous aider davantage.
Concentration
| Template | Concentration |
| Plasmid | 40-100 ng/µl |
|
PCR (purified and unpurified products)*
Length 100 - 400 bp
Length 401 - 900 bp
Length 901 - 2000 bp
|
General Rule: 1.5 ng/µl per 100 bp
2 - 6 ng/µl
4 - 17 ng/µl
12 - 70 ng/µl
|
| E.coli |
50 µl LB Medium OR
visible colony on agar plate
|
| E.coli (Ecoli NightSeq®) |
add 1 colony (diameter 1 mm) OR
add 5 µl turbid liquid culture |
| Plasmid (Full PlasmidSeq) |
20 ng/µl
|
| Genomic DNA (BacterialSeq) |
50 ng/µl
|
*La purification PCR en amont est fortement recommandée mais peut facilement être sous-traitée à Microsynth.
|
Primer
|
Concentration
|
|
Premixed
|
4 pmol/µl = 4 µM |
| As separate tube (enclosed) | 10 pmol/µl = 10 µM |
Volumes
| Service | Volume | Additional Information |
|
Economy Run (Tube)
|
12 µl
|
Premixed option: 12 µl DNA template solution + 3 µl 20µM sequencing primer solution
|
|
Economy Run (Plate)
|
10 µl/well in 96-well plate
|
Increase the volume by 5 µl /well for each additional reaction
|
|
Premium Run
|
At least 20 µl
|
Increase the volume by 5 µl for each additional reaction
|
|
Primer Walking Service
|
At least 20 µl
|
Single Stranded 0.5 µg of plasmid-DNA for every kb of your construct
Double stranded 1 µg of plasmid-DNA for every kb of your construct |
|
Full PlasmidSeq
|
At least 15 µl
|
Suitable plasmid sizes range from 2.5 kb to 25 kb. While plasmids ranging from 25 kB to 50 kb have not been tested yet, you may try them at your own risk. |
|
BacterialSeq
|
At least 20 µl
|
High Quality DNA is needed for this services. That means high-molecular weight DNA with no fragments <5 kb. |
| Primer | Volume |
| As separate tube (enclosed) | 20 µl + 3 µl per additional reaction |
Stockage
|
Service
|
Storage at Microsynth |
|
Economy Run (Tube)
|
4 days
|
|
Economy Run (Plate)
|
1 month
|
|
Premium Run
|
3 months
|
|
Primer Walking Service
|
3 months
|
|
Full PlasmidSeq (Tube)
|
4 days
|
|
Full PlasmidSeq (Plate)
|
1 month
|
|
BactSeq (Tube)
|
1 month
|
|
Primer (stored)
|
4 months
|
|
Primer (custom primer list)
|
10 months
|