Back to top
Nucleobase Modifications (für ASOs und siRNAs)
| Nucleobase Modifications | Position | ||
| 5’ | 3’ | int | |
| 5-Me-dC | X | ||
5-Methyl-2'-deoxycytidine
5-Methyl-2'-deoxycytidine when substituted for dC will increase the Tm by as much as 0.5°C per insertion. In addition, the presence of 5-Methyl dC in CpG motifs can prevent or limit unwanted immune responses that otherwise occur if oligos are administered in vivo, which is of particular importance in antisense applications.
|
Position
|
Synthesis Scale [µmol]
|
Purification
|
|||||
|
|
0.04
|
0.2
|
1.0
|
15
|
Des
|
HPLC
|
PAGE
|
| Int |
X
|
X
|
O |
X
|
X | ||
X = available/on stock
O = on request